7YV5

genetically encoded pH sensor Lime at pH6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	98
Detector technology	PIXEL
Collection date	2019-01-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	108.992, 108.992, 49.928
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.190 - 2.850
Rwork	0.205
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yv3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.190	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	8222	813
<I/σ(I)>	14.13
Completeness [%]	99.8
Redundancy	13.4
CC(1/2)	1.000	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium chloride 0.1 M MES pH6.0 20% w/v PEG 6000