7YUK

Complex structure of BANP BEN domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	36.965, 129.900, 40.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.170 - 2.110
R-factor	0.2266
Rwork	0.225
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yug
RMSD bond length	0.008
RMSD bond angle	1.482
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.086	0.471
Number of reflections	11802	927
<I/σ(I)>	17	4.5
Completeness [%]	99.9	99.4
Redundancy	12.4	12.2
CC(1/2)	0.998	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% w/v PEG3350; 0.02 M Sodium/potassium phosphate; 0.1 M Bris-Tris propane pH 6.5