7YUG

Structure of human BANP BEN domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	39.650, 50.041, 54.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 1.100
R-factor	0.1256
Rwork	0.124
R-free	0.14460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold structure prediction
RMSD bond length	0.019
RMSD bond angle	1.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.095	0.307
Number of reflections	44514	2190
<I/σ(I)>	45.45	9.92
Completeness [%]	99.9	99.6
Redundancy	12.4	12.3
CC(1/2)	0.995	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% v/v PEG500*MME;10% w/v PEG 20000,0.09 M Halogens, and 0.1 M Tris(base); BICINE pH 8.5