7YUE

Epitope-directed anti-SARS CoV 2 scFv engineered against the key spike protein region.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-09-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 2 2 21
Unit cell lengths	99.596, 129.900, 40.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.571 - 2.350
R-factor	0.1812
Rwork	0.178
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dsi
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.571	2.434
High resolution limit [Å]	2.350	2.350
Number of reflections	11051	899
<I/σ(I)>	19.32
Completeness [%]	97.3
Redundancy	11
CC(1/2)	0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	0.2 M sodium thiocyanate at pH 5.9 20% PEG 1000