7YUA
Structural Insight into a Metal-Dependent Mutase MtdL Revealing an Arginine Residue Covalently Mediated Interconversion between Nucleotide-Based furanose and pyranose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.843, 155.155, 94.636 |
| Unit cell angles | 90.00, 97.45, 90.00 |
Refinement procedure
| Resolution | 46.919 - 2.500 |
| R-factor | 0.1825 |
| Rwork | 0.179 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7YU9 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.910 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.085 | 0.412 |
| Number of reflections | 58110 | 4540 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | containing 18 % PEG 3350, 0.2 M Potassium Formate |
