7YU2
Structure of 6-aminohexanoate-oligomer hydrolase NylC, D122G/H130Y/T267C mutant, hydroxylamine-treated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.270, 144.022, 128.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.145 - 1.210 |
| Rwork | 0.116 |
| R-free | 0.14280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axg |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.105 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.062 | 0.650 |
| Rmeas | 0.068 | 0.745 |
| Number of reflections | 194470 | 9026 |
| <I/σ(I)> | 26.7 | 2.04 |
| Completeness [%] | 99.3 | 93.2 |
| Redundancy | 7 | 4.1 |
| CC(1/2) | 0.999 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 1.4 M ammonium sulfate, 0.2 M sodium chloride, 0.1 M HEPES pH 7.5. |
