7YTT
Crystal structure of vaccinia virus G3/L5 sub-complex (SeMet-labeled, P21 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.277, 41.919, 53.184 |
| Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
| Resolution | 29.248 - 1.810 |
| R-factor | 0.1985 |
| Rwork | 0.198 |
| R-free | 0.21110 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.744 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.113 | 0.544 |
| Number of reflections | 11689 | 1143 |
| <I/σ(I)> | 15.87 | 2 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.2 M Ammonium nitrate (pH 6.2), 20% w/v Polyethylene glycol 3350 |
