7YTO
The Crystal Structure Analysis of Creatine Amidinohydrolase from Alcaligenes sp. KS-85
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-26 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 0.978520 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 194.946, 113.761, 111.482 |
Unit cell angles | 90.00, 94.90, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.310 |
R-factor | 0.2155 |
Rwork | 0.215 |
R-free | 0.25690 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.350 |
High resolution limit [Å] | 2.310 | 2.310 |
Number of reflections | 105747 | 5251 |
<I/σ(I)> | 3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298.15 | 15%(w/v) PEG 6000, 100mM Citric acid/Sodium hydroxide |