7YTO

The Crystal Structure Analysis of Creatine Amidinohydrolase from Alcaligenes sp. KS-85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.978520
Spacegroup name	C 1 2 1
Unit cell lengths	194.946, 113.761, 111.482
Unit cell angles	90.00, 94.90, 90.00

Refinement procedure

Resolution	40.000 - 2.310
R-factor	0.2155
Rwork	0.215
R-free	0.25690
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.484
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.350
High resolution limit [Å]	2.310	2.310
Number of reflections	105747	5251
<I/σ(I)>	3
Completeness [%]	99.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298.15	15%(w/v) PEG 6000, 100mM Citric acid/Sodium hydroxide