7YTB
Crystal structure of Kin4B8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 100.030, 100.030, 116.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.270 - 3.000 |
| R-factor | 0.2185 |
| Rwork | 0.216 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.680 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.517 | 8.544 |
| Rmeas | 0.528 | 8.736 |
| Rpim | 0.105 | 1.733 |
| Number of reflections | 13912 | 1332 |
| <I/σ(I)> | 10.25 | 0.93 |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 23 | 24 |
| CC(1/2) | 0.994 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | PEG550MME, potassium thiocyanate, HEPES-NaOH |
