7YTB

Crystal structure of Kin4B8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 3 2 1
Unit cell lengths	100.030, 100.030, 116.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.270 - 3.000
R-factor	0.2185
Rwork	0.216
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.270	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.517	8.544
Rmeas	0.528	8.736
Rpim	0.105	1.733
Number of reflections	13912	1332
<I/σ(I)>	10.25	0.93
Completeness [%]	99.5	98.3
Redundancy	23	24
CC(1/2)	0.994	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	PEG550MME, potassium thiocyanate, HEPES-NaOH