7YTA

crystal structure of NtAGDP3 AGD1-2 in complex with an H3K9me2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	70.741, 70.741, 217.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.470 - 2.310
R-factor	0.2487
Rwork	0.247
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zwx
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.090	0.038	0.979
Rmeas	0.092	0.039	1.003
Rpim	0.019	0.008	0.215
Total number of observations	629775
Number of reflections	25568	2810	2505
<I/σ(I)>	3.9
Completeness [%]	100.0	99.9	100
Redundancy	24.6	23.1	21
CC(1/2)		0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	25% PEG 1500, 0.1 M sodium chloride and 0.1 M bis-Tris propane, pH 9.0