7YSY
Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) co-crystallized with UDP-N-acetylgalactosamine from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-03-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.166, 76.342, 80.023 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 32.020 - 2.160 |
| R-factor | 0.161 |
| Rwork | 0.158 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ys8 |
| RMSD bond length | 0.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.176 | 0.798 |
| Rmeas | 0.190 | 0.880 |
| Rpim | 0.072 | 0.364 |
| Total number of observations | 226949 | |
| Number of reflections | 33268 | 1655 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.8 | 5.6 |
| CC(1/2) | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000 |
