7YSY

Crystal Structure of UDP-glucose 4-epimerase (Rv3634c) co-crystallized with UDP-N-acetylgalactosamine from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-03-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	51.166, 76.342, 80.023
Unit cell angles	90.00, 91.80, 90.00

Refinement procedure

Resolution	32.020 - 2.160
R-factor	0.161
Rwork	0.158
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ys8
RMSD bond length	0.005
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.160	2.160
Rmerge	0.176	0.798
Rmeas	0.190	0.880
Rpim	0.072	0.364
Total number of observations	226949
Number of reflections	33268	1655
<I/σ(I)>	5
Completeness [%]	100.0	99.8
Redundancy	6.8	5.6
CC(1/2)		0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2M Sodium acetate trihydrate, 0.1M Tris pH 8.5 30% PEG 4000