7YSL

Crystal structure of D-Cysteine desulfhydrase with a trapped PLP-pyruvate geminal diamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	68.380, 85.478, 123.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.210 - 2.020
R-factor	0.1735
Rwork	0.172
R-free	0.20860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d97
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.090	2.070
High resolution limit [Å]	2.020	2.020
Rmerge	0.106	0.920
Number of reflections	46857	3518
<I/σ(I)>	13	2.1
Completeness [%]	97.4	99.8
Redundancy	7.5	7
CC(1/2)	0.998	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Carboxylic acids (sodium formate, ammonium acetate, sodium citrate, potassium sodium tartrate, sodium oxamate), 0.1 M imidazole/MES pH 6.5, 30% (v/v) PEG 500 MME/PEG 20000