7YRI

S-adenosylmethionine sensor upstream of mTORC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07
Spacegroup name	P 1 21 1
Unit cell lengths	64.080, 78.892, 98.892
Unit cell angles	90.00, 96.17, 90.00

Refinement procedure

Resolution	49.570 - 2.610
R-factor	0.2284
Rwork	0.225
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold2
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.570	2.730
High resolution limit [Å]	2.610	2.610
Number of reflections	29881	3638
<I/σ(I)>	7.2
Completeness [%]	99.6
Redundancy	6.7
CC(1/2)	0.948	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	Octyl-beta-Glucoside, Sodium citrate tribasic dihydrate, PGE 3350