7YRE
Crystal structure of a bright green fluorescent protein (StayGold) with triple mutations (N137A, Q140S, Y187F) in jellyfish Cytaeis uchidae from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18062 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.880, 86.938, 72.741 |
| Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
| Resolution | 43.507 - 2.300 |
| Rwork | 0.197 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF2 predicted |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.038 | 0.153 |
| Number of reflections | 35613 | 3489 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M Lithium chloride, 0.1 M Citrate pH4.0, 20% PEG6000 |
