7YQS

Neutron structure of a L-rhamnose-alpha-1,4-D-glucuronate lyase from Fusarium oxysporum 12S, L-Rha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.780, 65.800, 108.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 1.800
R-factor	0.1634
Rwork	0.162
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7esk
RMSD bond length	0.007
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.420	1.860	1.270
High resolution limit [Å]	1.250	1.800	1.250
Rmerge	0.059	1.174	0.886
Rpim	0.025	0.507	0.380
Number of reflections	115388	3825	5667
<I/σ(I)>	15	1.6	2.2
Completeness [%]	100.0	98	100
Redundancy	6.4	5.9	6.4
CC(1/2)	0.999	0.411	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Protein solution : protein 24mg/ml, 20mM Tris-DCl (pD 8.0), Reservoir solution : 33 % (w/v) PEG 1500, 0.1 M Tris-DCl (pD 8.5), 0.1 M L-Rha
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Protein solution : protein 24mg/ml, 20mM Tris-DCl (pD 8.0), Reservoir solution : 33 % (w/v) PEG 1500, 0.1 M Tris-DCl (pD 8.5), 0.1 M L-Rha