7YQR

Crystal Structure of Xcc NAMPT and its complex with NAM

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	115.950, 115.950, 317.750
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.850 - 2.000
R-factor	0.1883
Rwork	0.187
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yqq
RMSD bond length	0.007
RMSD bond angle	0.801
Data reduction software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.850	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	86028	6228
<I/σ(I)>	18.8
Completeness [%]	100.0
Redundancy	38.6
CC(1/2)	0.999	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	ammonium tartrate, N,N-Bis(3-D-gluconamidopropyl) cholamide, N,N-Dimethyldecylamine-N-oxide