7YQP

Xcc Nicotinamide Phosphoribosyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 62 2 2
Unit cell lengths	115.898, 115.898, 160.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.490 - 2.088
R-factor	0.2211
Rwork	0.218
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7yqo
Data reduction software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.620	9.300
High resolution limit [Å]	2.080	2.080
Number of reflections	25813	2831
<I/σ(I)>	21.8
Completeness [%]	100.0
Redundancy	14.05
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	ammonium tartrate, N,N-Bis(3-D-gluconamidopropyl) cholamide, N,N-Dimethyldecylamine-N-oxide