7YQG

Functional and Structural Characterization of Norovirus GII.6 in Recognizing Histo-blood Group Antigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	62.631, 94.597, 108.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.299 - 1.698
R-factor	0.1443
Rwork	0.143
R-free	0.17460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rlz
RMSD bond length	0.006
RMSD bond angle	0.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.698	1.700
Rmerge	0.072	0.072
Number of reflections	72108	71619
<I/σ(I)>	39.868
Completeness [%]	99.3
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291.15	0.2 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate pH 4.6, 20% (vol/vol) 2-propanol