7YQB

Functional and Structural Characterization of Norovirus GII.6 in Recognizing Histo-blood Group Antigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	62.444, 94.777, 109.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.389 - 1.704
R-factor	0.1735
Rwork	0.172
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rlz
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.100
Number of reflections	71255	66173
<I/σ(I)>	24.843
Completeness [%]	92.7
Redundancy	10.9
CC(1/2)		0.100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M calcium chloride dihydrate, 0.1 M sodium acetate trihydrate pH4.6, 20% (vol/vol) 2-propanol