7YQ2

Crystal structure of photosystem II expressing psbA2 gene only

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	122.135, 228.172, 286.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.1528
Rwork	0.151
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wu2
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	5.000	2.010
High resolution limit [Å]	1.900	4.000	1.900
Rmerge	0.089	0.039	1.297
Rmeas	0.100	0.044	1.453
Number of reflections	621644	32749	95564
<I/σ(I)>	11.03	32.61	1.35
Completeness [%]	99.6	99.6	99.7
Redundancy	4.9	4.685	4.807
CC(1/2)	0.998	0.998	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	285	5% PEG 1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM Mes BUFFER, pH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPARATURE 285K