7YPM
Crystal structure of transaminase CC1012 complexed with PLP and L-alanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.968, 79.272, 173.845 |
| Unit cell angles | 90.00, 100.31, 90.00 |
Refinement procedure
| Resolution | 46.606 - 1.984 |
| R-factor | 0.2171 |
| Rwork | 0.216 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gwi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.776 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.984 | 2.000 |
| Number of reflections | 117255 | 5815 |
| <I/σ(I)> | 11.762 | 3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.936 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.69 | 291 | 100 mM Tris-HCl, pH 8.69, 0.8 M LiCl, 29% (w/v) PEG 4000 |
