7YO9

Crystal structure of fusion protein of human TP53INP2 LIR and human GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-24
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 41
Unit cell lengths	46.390, 46.390, 75.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.390 - 1.750
R-factor	0.195
Rwork	0.194
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.006
RMSD bond angle	0.793
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.392	46.392	1.860
High resolution limit [Å]	1.750	5.220	1.750
Rmerge	0.091	0.087	0.804
Rmeas	0.106	0.102	0.948
Number of reflections	31600	1199	5051
<I/σ(I)>	7.66	15.83	1.14
Completeness [%]	99.5	99.3	97.9
Redundancy	3.554	3.643	3.552
CC(1/2)	0.989	0.985	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	10% 2-propanol, 0.1 M sodium phosphate/citric acid pH 4.2, 0.2 M lithium sulfate