7YNX

Crystal structure of Pirh2 bound to poly-Ala peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.282770
Spacegroup name	P 21 21 21
Unit cell lengths	69.470, 80.270, 84.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.520 - 2.300
R-factor	0.2201
Rwork	0.216
R-free	0.26360
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.520	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.247	1.328
Number of reflections	21662	2119
<I/σ(I)>	8.62
Completeness [%]	81.1
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M HEPES pH7.5, 0.1 M NaCl, 1.6 M Ammonium Sulfate