7YMQ

Crystal structure of lysoplasmalogen specific phopholipase D, F211L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	111.570, 93.750, 147.810
Unit cell angles	90.00, 90.36, 90.00

Refinement procedure

Resolution	46.880 - 2.290
R-factor	0.1875
Rwork	0.185
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pz0
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	2.430
High resolution limit [Å]	2.290	2.290
Number of reflections	129674	21072
<I/σ(I)>	5.7
Completeness [%]	94.4
Redundancy	3.5
CC(1/2)	0.987	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	PEG3350, Na Citrate, Ammonium Acetate