7YMB

Structure of Alcohol dehydrogenase from Candida glabrata(CgADH)complexed with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	150
Detector technology	PIXEL
Collection date	2021-07-29
Detector	Bruker PHOTON II
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	50.644, 48.322, 70.475
Unit cell angles	90.00, 96.63, 90.00

Refinement procedure

Resolution	24.590 - 1.800
R-factor	0.1872
Rwork	0.185
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b6k
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.590	1.890
High resolution limit [Å]	1.739	1.850
Rmerge		0.289
Number of reflections	32695	1678
<I/σ(I)>	10.3
Completeness [%]	95.0
Redundancy	2.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2M Ammonium acetate, 0.1M sodium acetate pH 4.6, 30% PEG 4000