7YLS

Structure of a bacteria protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 2 3
Unit cell lengths	135.613, 135.613, 135.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.610 - 1.800
R-factor	0.1526
Rwork	0.152
R-free	0.16810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
RMSD bond length	0.013
RMSD bond angle	1.233
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.610	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	77237	7635
<I/σ(I)>	17.93
Completeness [%]	100.0
Redundancy	26.8
CC(1/2)	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	2-Methyl-2,4-pentanediol, Sodium cacodylate trihydrate, Magnesium acetate tetrahydrate, 1,3-Propanediol