7YLD
Two monobodies recognizing the conserved epitopes of SARS-CoV-2 N antigen applicable to the broad COVID-19 diagnosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.551, 74.303, 87.451 |
| Unit cell angles | 108.51, 104.22, 96.67 |
Refinement procedure
| Resolution | 49.340 - 2.800 |
| R-factor | 0.2883 |
| Rwork | 0.286 |
| R-free | 0.34330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.519 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.730 | 2.660 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.092 | 0.515 |
| Number of reflections | 34998 | 1796 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 0.2M ZnSO4, 25% (v/v) MPD |
