7YL5
Cell surface protein YwfG protein complexed with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2019-05-15 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 43.312, 273.164, 322.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.569 - 3.000 |
| Rwork | 0.227 |
| R-free | 0.29550 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7yl4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.279 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.145 | 1.053 |
| Rmeas | 0.151 | 1.097 |
| Rpim | 0.042 | 0.304 |
| Number of reflections | 39549 | 5675 |
| <I/σ(I)> | 16 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.7 |
| CC(1/2) | 0.998 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate (pH 5.0-5.6), 1.3 M Li2SO4, 2% (w/v) polyethylene glycol 3350 |
