7YKV

The structure of archaeal nuclease RecJ2 from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	83.481, 175.395, 66.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.490 - 2.040
R-factor	0.17353
Rwork	0.171
R-free	0.22557
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.432
Data reduction software	HKL-3000
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	175.400	2.090
High resolution limit [Å]	2.040	2.040
Rmerge	0.147	1.105
Rmeas	0.158	1.189
Rpim	0.058	0.434
Total number of observations	456615	33745
Number of reflections	62817	4576
<I/σ(I)>	12.1	2.3
Completeness [%]	100.0
Redundancy	7.3	7.4
CC(1/2)	0.998	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% PEG 400, 50mM HEPES pH 7.6