7YKG

Crystal structure of MAGI2 PDZ0-GK/pSGEF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97737
Spacegroup name	P 21 21 21
Unit cell lengths	67.894, 79.700, 93.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.520 - 2.160
R-factor	0.2099
Rwork	0.206
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eeh 5ypo
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.160	5.830	2.160
Rmerge	0.102	0.054	0.594
Rmeas	0.111	0.059	0.647
Rpim	0.043	0.023	0.252
Number of reflections	27918	1534	1363
<I/σ(I)>	3.8
Completeness [%]	99.9	99.8	99.6
Redundancy	6.4	6	6.3
CC(1/2)		0.998	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M sodium chloride, 0.1 M Tris (pH 8.5), 25% w/v PEG 3350