7YKA

Crystal structure of Fis1 (Mitochondrial fission 1 protein)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	44.822, 56.283, 57.012
Unit cell angles	90.00, 100.06, 90.00

Refinement procedure

Resolution	39.750 - 2.300
R-factor	0.2274
Rwork	0.226
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nzn
RMSD bond length	0.001
RMSD bond angle	0.297
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.689
Number of reflections	12419	830
<I/σ(I)>	15.6	2.4
Completeness [%]	99.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	100 mM Imidazole pH 8.0 200 mM Zinc acetate 20% PEG 3000