7YK4
ox40-antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-21 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 0.9979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 124.769, 124.769, 194.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.730 - 2.700 |
| R-factor | 0.2363 |
| Rwork | 0.233 |
| R-free | 0.29850 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.087 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 2.797 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.154 | 0.992 |
| Number of reflections | 40452 | 40392 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 94.2 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M potassium sodium tartrate, 14% PEG 3350, 10% glycerol |
