7YK4
ox40-antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-12-21 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 0.9979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.769, 124.769, 194.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.730 - 2.700 |
R-factor | 0.2363 |
Rwork | 0.233 |
R-free | 0.29850 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.087 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.154 | 0.992 |
Number of reflections | 40452 | 40392 |
<I/σ(I)> | 9.5 | |
Completeness [%] | 94.2 | |
Redundancy | 9.5 | |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M potassium sodium tartrate, 14% PEG 3350, 10% glycerol |