7YJI
Crystal structure of Lpg1083 from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 93.660, 93.660, 136.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.296 - 2.100 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.23330 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.185 | 77.185 | 2.210 |
| High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
| Rmerge | 0.078 | 1.126 | |
| Rmeas | 0.162 | 0.081 | 1.163 |
| Rpim | 0.041 | 0.022 | 0.289 |
| Total number of observations | 268633 | 9246 | 41671 |
| Number of reflections | 17123 | 658 | 2590 |
| <I/σ(I)> | 12.3 | 25.5 | 2.9 |
| Completeness [%] | 94.7 | 99.9 | 100 |
| Redundancy | 15.7 | 14.1 | 16.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 287 | 0.1 M sodium acetate, pH 4.6, 2.4 M Sodium chloride |
