7YIK
The structure of archaeal nuclease RecJ2 from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.522, 177.818, 66.297 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.636 - 2.191 |
| R-factor | 0.1974 |
| Rwork | 0.195 |
| R-free | 0.24980 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.640 | 29.640 | 2.250 |
| High resolution limit [Å] | 2.190 | 9.800 | 2.190 |
| Rmerge | 0.085 | 0.035 | 0.794 |
| Rmeas | 0.089 | 0.037 | 0.823 |
| Rpim | 0.024 | 0.011 | 0.217 |
| Total number of observations | 6849 | 48817 | |
| Number of reflections | 99462 | 633 | 3504 |
| <I/σ(I)> | 22.2 | 51.8 | 3.4 |
| Completeness [%] | 99.1 | 92.8 | 90.9 |
| Redundancy | 14.1 | 10.8 | 13.9 |
| CC(1/2) | 0.999 | 0.999 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG 400, 50mM HEPES pH 7.6 |
