7YIK

The structure of archaeal nuclease RecJ2 from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	85.522, 177.818, 66.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.636 - 2.191
R-factor	0.1974
Rwork	0.195
R-free	0.24980
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.879
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.640	29.640	2.250
High resolution limit [Å]	2.190	9.800	2.190
Rmerge	0.085	0.035	0.794
Rmeas	0.089	0.037	0.823
Rpim	0.024	0.011	0.217
Total number of observations		6849	48817
Number of reflections	99462	633	3504
<I/σ(I)>	22.2	51.8	3.4
Completeness [%]	99.1	92.8	90.9
Redundancy	14.1	10.8	13.9
CC(1/2)	0.999	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12% PEG 400, 50mM HEPES pH 7.6