7YIC
Crystal structure of reductase LSADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-02-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.280, 109.928, 120.668 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.160 |
| R-factor | 0.297 |
| Rwork | 0.296 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.295 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Number of reflections | 24544 | 10327 |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 97.7 | |
| Redundancy | 2 | |
| CC(1/2) | 0.750 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | peg 3350 |
