7YHR

Anti-CRISPR protein AcrIC5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2021-10-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	54.250, 54.250, 42.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.490 - 1.450
R-factor	0.2115
Rwork	0.210
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gm6
RMSD bond length	0.007
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.490	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.085	2.248
Number of reflections	12804	1274
<I/σ(I)>	19.51
Completeness [%]	99.9
Redundancy	20.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.6 M sodium citrate tribasic digydrate pH6.5