7YHL
Crystal Structure of the ring nuclease Sso2081 from Saccharolobus solfataricus in complex with free phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-01 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.027, 38.376, 69.798 |
| Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
| Resolution | 40.950 - 2.700 |
| Rwork | 0.263 |
| R-free | 0.28770 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.802 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.950 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.067 | 0.748 |
| Number of reflections | 9099 | 924 |
| <I/σ(I)> | 10.9 | 1.5 |
| Completeness [%] | 95.5 | 95.55 |
| Redundancy | 5.29 | 5.32 |
| CC(1/2) | 0.998 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.1 M Sodium acetate trihydrate, pH 4.5, 25% w/v Polyethylene glycol 1500 |
