7YHL

Crystal Structure of the ring nuclease Sso2081 from Saccharolobus solfataricus in complex with free phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	67.027, 38.376, 69.798
Unit cell angles	90.00, 106.41, 90.00

Refinement procedure

Resolution	40.950 - 2.700
Rwork	0.263
R-free	0.28770
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.802
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.950	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.067	0.748
Number of reflections	9099	924
<I/σ(I)>	10.9	1.5
Completeness [%]	95.5	95.55
Redundancy	5.29	5.32
CC(1/2)	0.998	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M Sodium acetate trihydrate, pH 4.5, 25% w/v Polyethylene glycol 1500