7YHJ
Effector binding domain of LysR-Type transcription factor LrhA from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.682, 169.312, 190.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.713 - 3.237 |
| R-factor | 0.3217 |
| Rwork | 0.321 |
| R-free | 0.33760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onm |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.246 | 1.385 |
| Rmeas | 0.462 | 2.385 |
| Rpim | 0.172 | 0.890 |
| Number of reflections | 43674 | 4278 |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 5.1 | 5.3 |
| CC(1/2) | 0.977 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, 0.1 M HEPES, 0.2 M Ammonium sulfate |
