7YHJ

Effector binding domain of LysR-Type transcription factor LrhA from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	84.682, 169.312, 190.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.713 - 3.237
R-factor	0.3217
Rwork	0.321
R-free	0.33760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3onm
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.350
High resolution limit [Å]	3.230	3.230
Rmerge	0.246	1.385
Rmeas	0.462	2.385
Rpim	0.172	0.890
Number of reflections	43674	4278
<I/σ(I)>	3.2
Completeness [%]	98.5	98.1
Redundancy	5.1	5.3
CC(1/2)	0.977	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, 0.1 M HEPES, 0.2 M Ammonium sulfate