7YGY

Crystal structure of the Zn2+-bound EFhd2/Swiprosin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.2851
Spacegroup name	I 2 3
Unit cell lengths	92.900, 92.900, 92.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.450 - 2.600
R-factor	0.2304
Rwork	0.229
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i2l
RMSD bond length	0.010
RMSD bond angle	1.184
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.670	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	4243	518
<I/σ(I)>	40.3
Completeness [%]	100.0
Redundancy	80.8
CC(1/2)	1.000	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES-NaOH pH 7.5, 25% (w/v) PEG 8000