7YGP
DNA duplex containing 5OHU-T base pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00835 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 36.672, 36.672, 54.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.787 - 1.997 |
| R-factor | 0.2194 |
| Rwork | 0.219 |
| R-free | 0.22540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.787 | 20.787 | 2.050 |
| High resolution limit [Å] | 1.997 | 8.940 | 2.000 |
| Rmerge | 0.026 | 0.025 | 0.396 |
| Rmeas | 0.032 | 0.032 | 0.486 |
| Number of reflections | 5348 | 54 | 417 |
| <I/σ(I)> | 17.44 | 36.94 | 2.07 |
| Completeness [%] | 95.1 | 83.1 | 92.9 |
| Redundancy | 2.655 | 2.593 | 2.703 |
| CC(1/2) | 0.999 | 0.998 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD, lithium chloride, hexammine cobalt chloride, Sodium cacodylate |
