7YGL
Crystal Structure of the ring nuclease Sso2081 from Saccharolobus solfataricus in complex with A4>p cleavage intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.510, 39.144, 74.088 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 28.910 - 2.500 |
| Rwork | 0.258 |
| R-free | 0.27860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yhl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.043 | 0.851 |
| Number of reflections | 13097 | 1285 |
| <I/σ(I)> | 32.22 | 1.86 |
| Completeness [%] | 99.9 | 98 |
| Redundancy | 6.4 | 5.8 |
| CC(1/2) | 0.997 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.1 M Sodium acetate trihydrate, pH 5.0, 20% w/v Polyethylene glycol 1500 |
