7YGI

Crystal structure of p53 DBD domain in complex with azurin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-05
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	144.839, 68.750, 83.847
Unit cell angles	90.00, 99.69, 90.00

Refinement procedure

Resolution	19.876 - 2.100
R-factor	0.2158
Rwork	0.213
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kmd 1e67
RMSD bond length	0.009
RMSD bond angle	0.966
Data reduction software	ADDREF
Data scaling software	ADDREF
Phasing software	AMPLE
Refinement software	PHENIX (V 1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.880	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	46000	227437
<I/σ(I)>	6.3
Completeness [%]	99.5
Redundancy	6.4
CC(1/2)	0.899	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.05M Na2HPO4+19.5% PEG3350