7YF2
Crystal structure of METTL9 in complex with unmethylated SLC39A5 peptide and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.838, 45.836, 106.415 |
| Unit cell angles | 90.00, 122.64, 90.00 |
Refinement procedure
| Resolution | 35.418 - 1.691 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model from alphaFold2 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.560 | 1.780 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 63898 | 63898 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.4 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.999 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BICINE pH 9.0, 2% 1,4-Dioxane, 10% w/v PEG 20000, 12% Glycerol, 0.01 M TCEP |
