7YEY

Structure of MmIGF2BP3-KH12 in complex with 8-mer RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	38.266, 62.271, 66.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.2042
Rwork	0.203
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gqe
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.115	0.752
Rmeas	0.118	0.798
Rpim	0.034	0.256
Number of reflections	14115	1369
<I/σ(I)>	22.6	2
Completeness [%]	99.5	97.2
Redundancy	11.3	7.4
CC(1/2)	0.972	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	containing 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000