7YEX
Structure of MmIGF2BP3 in complex with 8-mer RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 40.439, 64.194, 73.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7yew |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix-1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.131 | 0.943 |
| Rmeas | 0.136 | 1.017 |
| Rpim | 0.040 | 0.364 |
| Number of reflections | 13473 | 1275 |
| <I/σ(I)> | 20.833 | 1.833 |
| Completeness [%] | 99.6 | 97.2 |
| Redundancy | 11.2 | 7.3 |
| CC(1/2) | 0.991 | 0.428 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20%w/v Polyethylene glycol 8000 |
