7YEO

Crystal Structure of cystathionine gamma-synthase-like protein C23A1.14c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	117.390, 106.820, 82.810
Unit cell angles	90.00, 128.00, 90.00

Refinement procedure

Resolution	27.150 - 1.700
R-factor	0.1624
Rwork	0.161
R-free	0.18570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6g
RMSD bond length	0.012
RMSD bond angle	1.406
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.150	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.074	0.896
Rpim		0.356
Number of reflections	87191	8664
<I/σ(I)>	15.21	2.87
Completeness [%]	98.8	98.01
Redundancy	6.9	7.2
CC(1/2)	0.999	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	peg550, HEPES, magnesium chloride