7YE8
Crystal structure of SARS-CoV-2 refolded dimeric ORF9b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.673, 73.673, 176.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.290 - 3.010 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.29850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cme |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.065 | 1.392 |
| Number of reflections | 11680 | 302 |
| <I/σ(I)> | 46.615 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.1 | 19.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8M sodium phosphate monobasic monohydrate, 0.8M potassium phosphate monobasic, 0.1M sodium HEPES pH7.5 |
