7YDT

Crystal structure of mouse MPND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 21 2 21
Unit cell lengths	37.677, 62.838, 112.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.971 - 2.055
Rwork	0.230
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pbd
RMSD bond length	0.002
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.098
Number of reflections	17365	732
<I/σ(I)>	13.3
Completeness [%]	97.6
Redundancy	5.8
CC(1/2)		0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG4000, Bis Tris, ammonium acetate