7YDO
Crystal structure of Atg44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2019-10-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 82.364, 82.364, 113.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.710 - 1.580 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.22620 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 52606 | 13704 |
| <I/σ(I)> | 5.53 | |
| Completeness [%] | 97.1 | 82.7 |
| Redundancy | 11.7 | |
| CC(1/2) | 0.978 | 0.543 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG 3350, octyl-glucoside, sodium citrate |
