7YCW

Crystal Form 1 of Truncated Antitoxin ParD (2-54,containg RHH domain) from Pseudoalteromonas rubra

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979150
Spacegroup name	P 42 21 2
Unit cell lengths	61.836, 61.836, 129.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.780 - 2.200
R-factor	0.2204
Rwork	0.219
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7b22
RMSD bond length	0.008
RMSD bond angle	0.961
Data reduction software	XDS (Feb 5, 2021 BUILT=20210323)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.780	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.601
Rmeas	0.071	0.613
Rpim	0.015	0.119
Number of reflections	13454	1294
<I/σ(I)>	30.41	5.95
Completeness [%]	99.7	98.92
Redundancy	24.1	25.8
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.2 M Sodium acetate trihydrate,0.1 M Sodium citrate pH 5.5, 5 % w/v PEG 4000